Abstract: Microcystic aeruginosa was taken as the study material in order to investigate changes in fluorescence characteristics of intracellular dissolved organic matter (IDOM) during its growth process. The species was cultured in the water samples from Jingpo Lake, Xingkai Lake, Wudalianchi Lake, Songhua Lake, Dahuofang Reservoir in the northeast plains and mountainous lake regions. The fluorescence characteristics of intracellular dissolved organic matter (IDOM) of M. aeruginosa were analyzed by the excitation emission matrix (EEM) fluorescence spectroscopy during its growth process. The results showed that EEM fluorescence spectra of IDOM mainly have two peaks (i.e., peak S and peak T), which indicated that protein-like material, and the fluorescence intensity of peak S was higher than that of peak T. According to the fluorescence regional integration (FRI) method, the EEM of IDOM was divided into five regions (Ⅰ-Ⅴ regions). Percent Fluorescence Response (P_i,n) showed that P_Ⅰ+Ⅱ,n and P_Ⅳ,n had a complementary trend and the decreasing order of Normalized excitation-emission area volumes (Ф_T,n) was Jingpo Lake>Wudalianchi Lake>Xingkai Lake>Songhua Lake>Dahuofang Reservoir. On the 16th day of cultivation, the average of the maximum Ф_T,n (Jingpo Lake) was 9.76×10^-5AU·nm²/(mg/L), while the average of the minimum Ф_T,n (Dahuofang Reservoir) was 3.72×10^-5AU·nm²/(mg/L). Hierarchical cluster analysis (HCA) suggested that the total ten water samples could be divided into three groups. Group 1involved HJ1、HJ2, group 2involved HX1、LD1、JS1、LD2, and group 3involved HX2、JS2、HW2、HW1. Among the three groups, the group 1(Jingpo Lake) was more suitable than the other two groups for the growth of M. aeruginosa.