Abstract: The ecotoxical effects of chromium (Ⅵ) to Eisenia fetida in the artificial soil were investigated by standardized methods recommended from OECD guidelines such as acute toxicity, growth and reproduction. The results showed that:1) The 14-day LC₅₀ (median lethal concentrations) of chromium (Ⅵ) for E. fetida were 245.06mg/kg and the 95% confidence interval were 208.31-288.29mg/kg (dry weight). 2) Compared with the control group, the growth of E. fetida were promoted significantly (P<0.01) in lower concentrations of chromium (Ⅵ) (10-20mg/kg), while the body weight were inhibited significantly (P<0.01) in higher concentrations of chromium (Ⅵ) (80-160mg/kg) after the exposure duration of 28d. 3) The cocoon production and number of juvenile were inhibited with the increasing concentrations of chromium (Ⅵ), and the EC₅₀ (medium effective concentration) values of cocoon production and number of juvenile inhibition were 62.32(42.13-92.17) and 49.92(33.53-74.34) mg/kg, respectively. This study indicated that the values of growth and reproduction could be regarded as sensitive parameters for evaluating the ecotoxicity of chromium (Ⅵ) to E. fetida, and the value of number of juvenile was the most sensitive parameter.