Abstract: To select the microbial colony with the high oil-degrading ability, fourteen filamentous fungi strains were isolated from the crude-oil contaminated soil. By using ITS (Internal Transcription Sequence) analysis, nine strains were identified as Fusarium sp., two strains were as Bionectria sp., and the others were identified as Stachybotrys sp., Aspergillus sp., and Actinomucor sp., respectively. The mycelial growth rates on the solid medium were significantly different (P<0.01). The results showed that the strains could degrade different components of petroleum hydrocarbons by GC-MS (Gas Chromatography-Mass Spectrometry) analysis. The selected optimal strains were classified to two communities, in which one community consisted of the strain No.3, D2, D3and D52, and the other community was composed of the strain No.3, No.6, D2and D3. During 30days of crude oil degradation test of the strains in the liquid medium with the crude oil, the crude oil removal rate (CORR) of the strain D52was the highest (64.25%), the CORRs of both communities were respectively 74.55% and 72.64%, which communities have considerable potentials to be used in the bioremediation of crude-oil contaminated soil.