Abstract: Efficient denitrifying bacteria isolated from sediments were fixed with ceramsite and were used to simulate the release of the carbon source in nitrate-rich landscape water. Meanwhile, advantages of denitrifying bacteria immobilized with ceramsite on nitrate-N removal during preservation at room temperature were also addressed by comparing with sponge. The results demonstrated that the sediment could effectively release the carbon source in the simulated experiments. The immobilization of denitrifying bacteria with ceramsite was an effective method for nitrate-N removal from landscape water. After 2days denitrification, the removal rate of nitrate-N reached 98.1% and the removal rate of total nitrogen appeared to be 91.5%. The concentrations of nitrate-N decreased from 16.72mg/L to 0.19mg/L after 10days denitrification, and the removal rate was about 98.8%. The concentration of total nitrogen decreased from 17.89mg/L to 0.95mg/L after 10days denitrification, and the removal rate was about 94.7%. Denitrifying bacteria immobilized with ceramsite kept higher activities after 60days preservation at room temperature. The concentration of nitrate-N decreased from 37.28mg/L to 0.08mg/L, and the removal reached 99.8%. The results indicated that denitrifying bacteria immobilized with ceramsite could be effetive method to remove the nitrate-N in the waste water, and could be further used in waste water treatment in the future.