Abstract: The subtropical coniferous and broad-leaved mixed forests at Jinyun Mountain, Chongqing were selected to investigate temporal variation characteristics of soil respiration temperature sensitivity (Q₁₀), and the variation affected by soil temperature and soil moisture was further analyzed. The soil respiration rate was measured by LI-8100soil respiration carbon dioxide flux measurement system. The soil temperature (T₅) and soil moisture (W₅) at 5cm depth were observed by DDTWS-Ⅱtype of soil temperature and moisture logger from April to December in 2011. Subsequetly, variation rule of R_S and Q₁₀ were analyzed, and the effects of T₅ and W₅ on R_S and Q₁₀ were discussed by using simple models and binary mixed models. The results showed that:(1) Monthly average value of R_S and T₅ showed the curves with a single peak. R_S ranged from (1.38±0.15) to (3.94±0.21)μmol/(m²·s), while T₅ ranged from (9.28±0.65) to (22.99±1.14) ℃. The monthly variation of W₅ was not regular due to the precipitation. (2) Seasonal differences of Q₁₀ were apparent. The values of Q₁₀ showed a maximum at 3.31in spring and their average values during observation process was 2.01. (3) R_S showed a significant positive correlation (P<0.05) with T₅ but showed no significant correlation (P>0.05) with W₅.The goodness of fit of simple fitted model of R_S and T₅ was 87%, while that of simple fitted model of R_S and W₅ was 26%. The goodness of fit of the binary mixed model showed that the combined effects of T₅ and W₅ on R_S was 89%. The binary mixed model can better interpret the variation of R_S better then simple models. (4) T₅ was the main factor affecting Q₁₀ following by and the secondary factor, W₅. Compared to the simple model, the binary mixed model can more clearly explain the variation of Q₁₀.