Abstract: Pollutions of antibiotics in the rivers are rarely reported, although they are considered as important components of environmental pollution for aquatic organisms in the city sewage system in China. In order to understand the contaminated status of the Qing River in Beijing, we analyzed 4kinds of antibiotics in the water and aquatic organisms from this urban river. The concentrations and distributions of the 15antibiotics were tested and analyzed by high performance liquid chromatography and mass spectrometry. The results showed that quinolones and macrolides were the main components of pollutants in the water, with average concentrations of 2238.9and 814.9ng/L, respectively. As for the aquatic organisms, quinolones 945.3μg/kg and tetracyclines 389.2μg/kg were the main contents in the five aquatic plants, whereas quinolones 3213.9μg/kg and sulfonamides 653.5μg/kg were the main pollutants in fish and mollusks. In addition, the environmental risk assessment of antibiotics in aquatic ecosystems showed that the hazard quotients(HQs) of of loxacin, ciprofloxacin, and norfloxacin to the algae and aquatic plants were more than 1, this indicated that these three antibiotics pose an environmental risk to the health of algae and aquatic plants. In conclusion, a certain degree of antibiotic pollution existed in the Qing River, and the potential environmental risk should not be ignored, considering the relatively high HQs of quinolones.