氨氧化菌对填埋场CH4协同氧化及铵抑制作用的研究

Landfill CH4Co-Oxidation by Ammonia-Oxidizing Bacteria and the Inhibition Effect by Ammonium

  • 摘要: 借助乙炔(C2H2)抑制和添加外源铵盐,采用批式培养试验,在初始CH4浓度为16%的条件下模拟填埋场高CH4浓度环境,通过分析样品中氨氧化菌对CH4氧化的贡献率及铵对CH4氧化的抑制率,研究了填埋场覆盖土、矿化垃圾、砂土和黏土中氨氧化菌对CH4协同氧化及铵抑制作用. 结果表明:4种供试样品中氨氧化菌对CH4氧化的贡献率在5.64%~16.24%之间,次序为砂土<黏土<覆盖土<矿化垃圾,覆盖土中的贡献率为14.90%,比矿化垃圾低8.25%,填埋场样品(矿化垃圾和覆盖土)是一般土壤(砂土和黏土)的1.8~10.9倍. 铵对CH4氧化过程的抑制率在11.90%~24.84%之间,次序为砂土<黏土<覆盖土<矿化垃圾,覆盖土中为23.21%,比矿化垃圾低6.56%,填埋场样品是一般土壤的0.9~2.1倍. 填埋场样品中氨氧化菌对CH4氧化的贡献率及铵对CH4氧化的抑制率明显高于一般土壤.

     

    Abstract: Based on inhibitor acetylene (C2H2), ammonium addition and batch incubation, the contribution of ammonium-oxidizing bacteria to the process of landfill methane (CH4) oxidation and the inhibition effect by ammonium addition have been analyzed. Four different types of materials have been sampled:landfill covered soil, mineralized refuse, sandy soil and clay soil under the condition of the initial CH4concentration of 16% to simulate landfills environment. The contributions of ammonia-oxidizing bacteria to CH4oxidation ranged from 5.64% to 16.24% and following the followed increasing order:sandy soil4oxidation were confirmed from 11.90% to 24.84% in the four tested samples, which followed the order as sandy soil4oxidation in landfill samples were significantly higher than those for sandy soil and clay soil.

     

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