Abstract: Acute toxicity and oxidation stress response of Eisenia fetida exposured to lead in soil was studied based on the test method recommended by OECD. Recently the biochemical toxicity of lead, particularly its influences on antioxi-dants in the earthworm is still limited. Thus, acute and biochemical biomarkers including mortality, superoxide dismutase(SOD), catalase(CAT), peroxidase(POD) and malondialdehyde (MDA) were applied in the test. The toxicological tests results showed that 14d LC₅₀ was 2576.76mg/kg on E. fetida under lead exposure in soil and the 95% confidence interval was 2388.42-2792.00mg/kg. The SOD activity in the 1^st, 7^th, 14^th,the POD activity in 1^st, 14^th and the content of MDA in the fourteenth day indicated a good dose-response relationship with the concentrations of lead pollution exposure, while CAT activity had not indicated dose-response relationship between activity and concentration. The sensitivities of the three kinds of antioxidant enzymes were SOD>POD>CAT on E. fetida under lead exposure in artificial soil. As an important antioxidant enzyme, SOD could effectively predict the subletal toxicity of lead on E. fetida.