Abstract: A batch experiment was conducted to investigate washing effects of four kinds of synthetic surfactants (i.e., triton X-100(TX-100), tween 80(TW-80), sodium dodecyl benzene sulfonate (SDBS), and cetrimonium bromide (CTMAB)), two species of biosurfactants (i.e., saponin and rhamnolipid fermentation liquor (RL)), and one biogenic product (i.e., β-cyclodextrins (β-CD)) for PCBs (Aroclor1254) contaminated soil. The results showed that the overall washing efficiencies of seven eluents on the PCBs contaminated soil followed the sequence of TX-100≈TW-80＞SDBS＞β-CD＞Saponin＞RL＞CTMAB. When the mass concentration of eluents was 10g/L, the elution rate of TX-100, TW-80, SDBS, β-CD, Saponin, RL, CTMAB could reach up to 54.99%, 53.12%, 46.99%, 25.35%, 14.76%, 13.70%, 0%, respectively. Taking the flushing efficiency, the soil adsorption amount, the cost of eluents and whether friendly to the environment into consideration, TX-100and β-CD were chosen as the two preferable flushing agents for PCBs contaminated soil and the influences of flushing time, elution times and the pH of these two eluents on the desorption were carried out. The results showed that the washing efficiency of TX-100and β-CD got the infection point and then the washing rate increased less after 12h elution, three times of flushing of the two selected eluents showed that the first time accounted for the major part and the second and third times contributed little to elution effects, the flushing efficiencies of the same volume of eluents divided into twice were similar to once, and at the same time, the washing efficiencies were no significant differences when the aqueous pH of the two selected eluents varied from 4to 10.