Abstract: Antibiotic resistant pathogenic bacteria pose a potential threat to human health. In order to investigate the antibiotic resistance of Salmonella in municipal wastewater, 81strains were isolated from two municipal wastewater treatment plants. The analysis of antibiotic resistance to tetracycline and sulfamethoxazole in these isolates was conducted by Kirby-Bauer disc agar diffusion method. The presence of antibiotic resistance genes, tetA, tetB, sul1, sul2and sul3, in antibiotic resistant Salmonella were investigated by the PCR method. The results showed that the antibiotic resistance rates to tetracycline and sulfamethoxazole were 53.1% and 40.7% respectively, and most of antibiotic resistant Salmonella showed a dual resistance. The rate of dual resistance to tetracycline and sulfamethoxazole in Salmonella isolated from effluent after chlorination was 30.7% higher than in isolates from raw wastewater. The genes tetA and sul3were frequently detected in antibiotic resistant Salmonella in the raw wastewater, while the distribution of antibiotic resistance genes in Salmonella changed significantly after wastewater treatment process. The results implicated that the prevalence of antibiotic resistance of Salmonella in municipal wastewater and effluent may be a major risk to human health.