Abstract: In order to investigate the effect of disinfection method on the antibiotic resistance of bacteria in the secondary effluent of municipal wastewater treatment plant, the secondary settling tank effluent samples were used to conduct the experiments of chlorination and ultraviolet disinfection. Ampicillin (AMP), tetracycline (TET), ciprofloxacin (CIP) and chloramphenicol (CHL) were chose as typical antibiotics to study the resistance rates of fecal coliforms before and after disinfection. The results showed that when the available chlorine concentration in range of 0-1.0mg/L during chlorination process, the fecal coliforms resistance rate to AMP, and CIP increased with the increase of chlorination dose after chlorination, while TET-resistant rate showed a downward trend. After treatments with 1.0mg/L chlorine, the resistance rates to AMP, TET, CIP, CHL were 35.1%, 5.6%, 62.3%, 0, respectively. Fecal coliforms can restore their vesistances to AMP, TET and CIP after chlorination. At 48h after chlorination, these resistance rates were higher than those before chlorination. When the ultraviolet irradiation dose was low (approximate under 16mJ/cm²), the fecal coliforms resistance rates to antibiotics decreased with the increase of ultraviolet irradiation dose, however, but the high dose of ultraviolet irradiation may cause the rise of the antibiotic resistance rate.