Abstract: Screening of sensitive test organisms plays a key role on deriving aquatic life criteria. Fish species is one of the important protection objects for the water quality criteria. The ecotoxicity data of fish are required for deriving water quality criteria in the guidelines of developed countries. In the present study, most of seventeen native representative fish species belonging to Cyprinidae were obtained based on their geographical distribution and quantity of the toxicity data. According to the data collecting principle of aquatic life criteria,the fish toxicity data were collected and screened from database of ECOTOX and published literatures. The list of most pollutants toxic to fish was obtained through species sensitivity analysis. The pollutants included pesticides, chlorphenols, heavy metals and ammonia. The species sensitivities of fish species were classified by the cumulative probability from the sensitivity distribution curves of the most toxic pollutants. Ten fishes of the seventeen species were selected to be the native test model organisms for deriving water quality criteria. The fishes included Cyprinus carpio (fenvalerate,12.50%; thiram, 14.29%), Ctenopharyngodon idellus (triazophos, 9.09%), Hypophthalmichthys molitrix (fenpropathrin, 12.50%), Aristichthys nobilis (cadmium, 7.14%; dichlorvos, 13.63%), Carassius auratus (inorganic mercury, 11.76%)belonging to Cyprinidae, and Misgurnus anguillicaudatus (dichlorvos, 9.09%), Pelteobagrus fulvidraco (dichlorvos, 4.55%; omethoate, 14.29%), Monopterus albus (cypermethrin, 8.33%), Mugilce phallus (endosulfan, 8.33%; fenvalerate, 6.25%) and Siniperca chuatsi (non-ionic ammonia, 9.09%) belonging to non-Cyprinidae. The screened species can be considered as the native sensitive fish species for development of aquatic life criteria of their corresponding pollutants.