Abstract: Amphibians are important biological community in aquatic ecosystems, and are one of the important protected objects of aquatic life criteria. The toxicity data of twelve representative freshwater amphibians were collected and screened according to the guidelines of the US for deriving water quality criteria. The analysis results showed that there were three categories of pollutants, which were the most toxic to amphibians, including pesticides, heavy metals and insecticides. Four genuses for development for water quality criteria were obtained. The calculated results of the cumulative probability showed that the Bufo melanostictu to silver nitrate was 15%, Rana hexadactyla to malathion was 1%, R. catesbeiana to chlorophenasicacid was 8%,Paa spinosa to phosphorothioic acid was 27%, and Hoplobatrachus tigerinus to endosulfan was 29%. The Rana (R. hexadactyla) to pesticide, Rana (R. catesbeiana) to insecticide and Bufo (B. melanostictu)to heavy metals were sensitive, respectively. The Hoplobatrachus (H. tigerinus) to pesticide and Paa (P. spinosa) to insecticide were relatively sensitive, respectively. The five amphibian species can be used as the test organisms for development of water quality criteria.