Abstract: There are many uncertainties about the interactions between natural organic matters and heavy metals, due to the diverse sources, structures and properties of natural organic matter. Nowadays more and more advanced analytical methods are used to study these interactions. This study reviewed the main analytical methods for investigating the interaction sbetween natural organic matter and heavy metals, including spectroscopy analysis, isothermal titration calorimetry, stripping voltammetry, zeta potential analysis and high performance size exclusion chromatography. The methods can investigate the interactions between natural organic matters and heavy metals from different aspects, including spectroscopic characteristics, zeta potential, molecular weight, thermodynamic parameters and electrochemical properties. Although research using these methods has made some progress, it is still insufficient. Because each individual analytical method has its own characteristic and limitations, it is suggested that a variety of methods can be combined to study the interaction between natural organic matters and heavy metals in future work. It is also suggested to create a multi-parameter dependent model to qualitatively and quantitatively analyze and predict the NOM-heavy metal interactions based on knowing the contribution of each parameter to the interactions.