Abstract: A gantry is used to fix printed circuit board (PCB) during processing, during which copper is plated on the surface of the gantry. The gantry can be reused after its surface is treated by acid solution, but this generates a large amount of waste solution and poor working conditions. In this study, an environmentally-friendly method based on a microbial technique was used to recycle Cu. A strain of Acidithiobacillus ferrooxidans was used in the experiment. The effects of bioleaching Cu from the gantry and its accessories and the composition ratio of 9K media (100% 9K medium, 50% 9K medium, 25% 9K medium) were systematically studied, and the leaching process was preliminarily analyzed. The results showed that when adding the 100% 9K medium, the concentrations of Cu²⁺ in the leaching solutions of the gantry and its accessories were the highest:2.31 and 1.06 g/L, respectively. Cu was removed totally from the surface of the gantry. When A. ferrooxidans leached copper from the gantry, the results showed that Fe²⁺ was oxidized to Fe³⁺ in the leaching solution, and the copper might be oxidized to Cu²⁺ with the action of Fe³⁺. Further nonline fit analysis proved that the pseudo-second order kinetic model was suitable for describing the relationship between the behavior of copper dissolved and bioleaching time. In summary, the bioleaching method is expected to be applied in the PCB industry.